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STEMCELL淋巴細(xì)胞分離液Lymphoprep

  • 更新時間:  2023-07-26
  • 產(chǎn)品型號:  07851
  • 簡單描述
  • STEMCELL淋巴細(xì)胞分離液Lymphoprep
    Lymphoprep分離液
    Lymphoprep是一種無菌、內(nèi)毒素含量低的即用型分離液,是目前國內(nèi)外分離正常人或病人外周血單個核細(xì)胞(PBMC:Mononuclear cells of Peripheral blood)(包括單核細(xì)胞monocytes和淋巴細(xì)胞lymphocytes)的常用方法,具有與昂貴的GE公司的PLUS或PREMIUM分
詳細(xì)介紹

STEMCELL淋巴細(xì)胞分離液Lymphoprep

*替代Ficoll-Paque

工作原理
PBMC與血液中的其他成分存在密度差異,利用密度在1.077±0.001g/ml之間而且近于等滲的LymphoprepTM溶液作密度梯度離心時,紅細(xì)胞和粒細(xì)胞由于密度大,故沉于分離液底部。PBMC密度稍低于分離液,故位于分離液界面上,獲得PBMC。LymphoprepTM分離液含有多糖(polysacccharide),多糖能粘附在細(xì)胞表面,使紅細(xì)胞和粒細(xì)胞聚集下沉,但它對淋巴細(xì)胞的活性和紅細(xì)胞和粒細(xì)胞進(jìn)一步分離有負(fù)面影響。

STEMCELL淋巴細(xì)胞分離液Lymphoprep

注意事項:
1、該分離液僅適用于人體的外周血液,不適用于動物。
2、使用該分離液時,需將待分離的血液用生理鹽水做等體積的稀釋。
3、分離液的保存應(yīng)置于無菌環(huán)境,并避光保存。
4、詳細(xì)操作步驟見產(chǎn)品說明書。
技術(shù)參數(shù)
成分:泛影葡胺鈉(Sodium diatrizoate):9.1% (w/v);多糖:5.7% (w/v)
密度:1.077±0.001g/ml
滲透性:290±15mOsm
內(nèi)毒素含量:<1.0EU/ml
分離所得PBMC中紅細(xì)胞的含量:整個細(xì)胞數(shù)目的3-10%。 
Lymphoprep™ is a density gradient medium recommended for the isolation of mononuclear cells from peripheral blood, cord blood, and bone marrow by exploiting differences in cell density. Granulocytes and erythrocytes have a higher density than mononuclear cells and therefore sediment through the Lymphoprep™ layer during centrifugation. Lymphoprep can be substituted for Ficoll-Paque™ without any need to change your existing protocols and is fully compatible with both Sepmate™ and RosetteSep™.

Product Type:Cell isolation products
Recommended For:

Isolation of mononuclear cells from peripheral blood, cord blood, and bone marrow

Components:

• Sodium Diatrizoate 9.1% (w/v)

• Polysaccharide 5.7% (w/v)

• Water

• Density of 1.077 g/mL

Accessory Products:

SepMate™-50 (Catalog #15450/15460)

Contains:

• Sodium Diatrizoate (9.1% w/v)

• Polysaccharide (5.7% w/v)

• Density of 1.077 g/mL

Equipment Required:

Centrifuge

Cell Isolation Product Type:Reagents
Cell Source:Bone marrow, Cord blood, Whole blood
Legal Statement:Lymphoprep™ is a trademark of Axis-Shield.
Intended Use Statement:FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement:STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.

訂購信息:

07851 500ml

07861 6*500ml

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